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Information × Registration Number 0221U102270, 0120U104577 , R & D reports Title Development of the scientific grounds for the biotechnological production of novel hypotensive and antiarrhythmic alkaloids from Rauwolfia serpentina Benth. popup.stage_title Head Kunakh Viktor A., Доктор біологічних наук Registration Date 29-01-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  A study was conducted on the cultivation of Rauwolfia serpentine tissue culture on different nutrient media differed in sugar content, at different temperatures and with different duration of subculture and time to harvesting. A productive K27M cell line has been created, which accumulates target antihypertensive and antiarrhythmic alkaloids. It is established that the optimal conditions for its cultivation are: nutrient medium with high sucrose content up to 10%, growing in the dark at a temperature of 27-28 °C and relative humidity of 65-75% for 40 days before subculture and 60-70 days before harvesting. Five batches of cell biomass in total of 1.5 kg with different content of alkaloids were accumulated. Key stages of isolation, purification, and identification of alkaloids from cellular biomass have been developed. HPLC methods were used to identify alkaline alkaloids; individual alkaloids were isolated and purified. In particular, 2 g of the antiarrhythmic alkaloid ajmaline and 8 g of total indoline alkaloids were isolated and purified. Product Description popup.authors Andrieiev Ihor O. Bieda Oleksandr A. Konvalyuk Iryna I. Lukashov Serhii S. Mozhylevska Ludmyla P. Yarmoluk Sergiy M. popup.nrat_date 2021-01-29 Close