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Information × Registration Number 0221U102318, 0120U102258 , R & D reports Title Development and implementation of an innovative method of molecular genetic bioindication of pesticide load popup.stage_title Head Gasso Viktor Ya., Кандидат біологічних наук Registration Date 01-02-2021 Organization Oles Honchar Dnipro National University popup.description2  Object of research: natural populations of animals and their experimental model groups. Research purpose: creation of methodical means and protocols of experiments for determination of molecular genetic markers of pesticide cytotoxicity, estimation of consequences of their action on the populations. Research methods and equipment: field ecological, polyacrylamide gel electrophoresis, chemiluminescence method. The expressed dose-dependent character of increase of protein RPA1 content in the rainbow trouts’ brain exposed to lambda-cyhalothrin (5% LC50 and 10% LC50) is revealed. There is a suppression of transcriptional activity and restriction of repair of DNA breaks. Increased expression of RPA1 may be an adequate biomarker of the toxic effects of synthetic pyrethroids in fish brain. An experimental research model is described. Pesticide treatment of agricultural fields leads to the suppression of herpetofauna populations in the adjacent forest shelter belts. 2 species of amphibians and 3 species of reptiles were found in the forest belts. The grass snake was found only in forest plantations remote from agrocenoses that were not treated with pesticides. The lowest species richness (0.48) is determined for the forest belts adjacent to agrocenoses. Artificial forest plantations that are affected by pesticides can be refugia to maintain biodiversity. For the first time the possible connection of peroxidation induced by pesticide intoxication on the processes of DNA replication and repair control in a model experiment was revealed; the influence of proximity to agricultural lands treated with pesticides on herpetofauna community was assessed. Sphere (branch) of use: activity of the Ministry of Environmental Protection and Natural Resources of Ukraine, theory and practice of nature protection, determination of consequences of ecosystem pollution for biota. Product Description popup.authors Yermolenko Sergyi V Bobilov Yuryi P Hahut Anna M. Holoborodko Kyrylo K Huslystyi Artem O Kulbachko Yurii L. Petrushevskyi Vladyslav B popup.nrat_date 2021-02-01 Close