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Information × Registration Number 0221U102411, 0116U003490 , R & D reports Title Establish mechanisms and create a physical-mathematical theory of cryoinjury cell under the influence of electrical voltages that occur during low-temperature preservation of cell suspensions. popup.stage_title Head Gordienko Olga I., Кандидат сільськогосподарських наук Registration Date 01-02-2021 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description2  The object of research is a physical-mathematical model that describes the transmembrane transfer of substances between cells and the environment during the process of low-temperature preservation and takes into account the influence of transmembrane potential, cooling rates, geometric and transport characteristics of cells. The aim is to elucidate the mechanisms and create a physical-mathematical theory of cells cryo-damage by electrical voltages that occur in the cell suspension during its low-temperature preservation, and to develop effective ways to prevent these damages. Research methods – physical-mathematical analysis and numerical simulation (computers), light microscopy, cryomicroscopy, morphometric analysis (cryomicroscope, confocal microscope). The physical-mathematical model of substances transmembrane transfer between cells and the environment is built, taking into account the electrical membrane potential. The computer program has been created for calculating the dynamics of changes in cell volume, cryoprotectant concentrations, basic ions and transmembrane potential during the cell exposure in cryoprotectant solution and freezing to eutectic temperatures. An algorithm for determining the filtration and permeability coefficients for cryoprotectants of cell membranes at subzero temperatures has been developed. The model of electrical breakdown of cell membranes is created, taking into account short-acting hydration repulsive forces between lipid bilayers. The physical-mathematical model based on the two-factor theory of cryodamage, thermodynamic theory of homogeneous crystallization and general theory of activation-type processes has been developed for determining the probability of cell damage by solution effects and intracellular crystallization to finding the optimal cooling rate. Product Description popup.authors Bondarenko Lyubov Ya. Goltsev Kyryl A. Kireev Vitaliy O. Kovalenko Igor F. Kovalenko Svitlana Ye Kuleshova Larysa G. Makedonska Valentyna O. Popivnenko Lyudmyla I. Smolyaninova Evgenia I. Tymofyeyeva Olena V Todrin Oleksandr F. Kholodnyy Vitaliy S popup.nrat_date 2021-02-01 Close