Search academic texts

Information × Registration Number 0221U102951, 0116U000668 , R & D reports Title To develop the molecular biotechnology for evaluation of maize genotypes resistance to extreme hydrothermal environmental factors popup.stage_title Head Fait Viktor I., Доктор біологічних наук Registration Date 12-02-2021 Organization Selection-Genetic Institute - National Center of Seed and Selection Studies of Ukrainian Academy of Agrarian Sciences popup.description2  Bioinformatics analysis of polymorphism of 23 genes / loci associated with root system architecture and hormone synthesis, ZmDREB1a and ZmDREB1c genes and their homologues examined the nature of the evolution of these genes: most genes were highly conserved, the nature of selection for these genes was stabilizing. For the susceptible TZEI16 line, the same levels of abscisic acid stress ripening1 (aasr1) gene expression were found in both osmotic stress (10% and 20% PEG 6000) and controls. In the DEN60 drought tolerant line, gene expression was not observed in the control, whereas under stress the expression level was twice (10% PEG 6000) and four times (20% PEG 6000) higher than the expression level of this gene in the susceptible line. Maize samples of different geographical origin, differing in the level of drought resistance, were analyzed by the genes ZmVPP1, LTP and the dehydrin genes (Dhn1 and ZmDhn13). Four different alleles of the ZmVPP1 gene and five different alleles of the Dhn1 locus were identified, differing in the size of the amplified product. Allele frequencies are determined. The predominance of certain alleles for drought-resistant and drought-sensitive genotypes was observed. Two alleles of the ZmDhn1 locus were detected: 88 bp. and 92 bp, which occurred with a frequency of 61.5 and 38.5%, respectively. These alleles were not associated with drought tolerance. Three different alleles 174, 177 and 187 bp were identified by the LTP gene (Lipid transfer protein). Drought resistant genotypes were characterized by a 174 bp allele, and drought sensitive genotypes were characterized by all three variants of alleles. Product Description popup.authors Galaeva Mariya V popup.nrat_date 2021-02-11 Close