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Information × Registration Number 0221U103137, 0116U003492 , R & D reports Title Studying the properties of polyvinyl alcohol as a recrystallization inhibitor in cryoprotective media based on endo and exocelular cryoprotectants. popup.stage_title Head Kompaniets Antonina M., Доктор медичних наук Registration Date 14-02-2021 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description2  The aim of the work is to experimentally substantiate the expediency of using polyvinyl alcohol in cryoprotective media of different composition to create new effective cryopreservatives. Object of study: PVA solutions (9 kDa, 31 kDa and 75 kDa), human erythrocytes. Research methods. Stalagmometry, light microscopy, laser confocal microscopy, cryomicroscopy, fluorescence spectroscopy, flow cytofluorimetry, optical spectroscopy, hematological, biochemical, physicochemical methods, molecular modeling, statistical analysis.Experimental grounding (reasoning) of the expedience of the usage of polyvinyl alcohol in cryoprotective media of varying composition for creation of new effective cryopreservatives. Research methods. Stalagmometry, optic microscopy, confocal microscopy, cryomicroscopy, fluorescence microscopy, flow cytometry, optic spectroscopy, hematological, biochemical, physicochemical methods, molecular modeling, statistical analysis. Research results. Physicochemical properties of water solutions of PVA of various molecular mass and combined cryoprotective media (CPM) that contained PVA were measured. The morphology of crystal structure of PVA solutions during warming was investigated. The role of cooling rate was assessed. Cytotoxic and cryoprotective efficiency of PVA solutions and CPM containing PVA were assessed under cooling human RBCs. The impact of biologically active substances on cryoprotective properties of PVA was esteemed. General patterns of CPM action that contained PVA were defined under low-temperature cryoconservation of biological objects. The expedience of the usage of combination of cryoprotectors with PVA in cryopreservative compositions for cooling biological objects was reasoned. Field of application are theoretic and applicable cryobiology, cryobiophysics, cryobiochemistry. Product Description popup.authors Gvozdyuk Yana V. Dyubko Tetyana S. Dyakonenko Ganna Yu. Kalashnikova Svitlana V. Ovsyannikov Sergiy E. Ostankova Liudmyla V. Pakhomova Yuliia S. Chekanova Valentina V popup.nrat_date 2021-02-14 Close