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Information × Registration Number 0221U103207, 0116U000269 , R & D reports Title To resaerch Campylobacter and Yersinia ecogeografic properties on territory of Ukraine and create modern system of diseases diagnosis and prevention popup.stage_title Head Zavhorodnii Andrii I, д.вет.н.Obukhovska Olena V, Registration Date 16-02-2021 Organization National Scientific Center Institute of Experimental and Clinical Medicine popup.description2 According to the results of the study of blood sera from animals from different regions of Ukraine, a high seroprevalence to the pathogens of campylobacteriosis and yersiniosis of animals was established: to yersinia infection among cattle (from 18.8% to 30% caused by Y. enterocolitica O3; from 6.3% to 23.9 % caused by Y. enterocolitica O6.30 and from 15.1% to 17.4% caused by Y. enterocolitica O9) and to the causative agent of genital campylobacteriosis (C. fetus ssp. venerealis) antibodies were detected in 32.9% of cases, and specific to Campylobacter C. fetus ssp. fetus - in 27.1% of animals. In 50% of cases, antibodies to each of these pathogens were detected. Molecular typing of the field and manufactural strains was performed, as a result of which it was established that epizootic strains of C. fetus by amplification of aspA and glnA genes were assigned to the subtype venerealis. Yersinia strains belong to the species Y. enterocolitica. Strain serotype O: 9 contains the gene ail, which indicates its virulence. All studied strains do not contain plasmid pYV-PL, which is likely to be associated with long passages at temperatures below 30 C0. In order to create DNA reference standards, phenotypically typical strains of C. fetus subsp. fetus LBF (C. f. f. LBF) and C. fetus subsp. venerealis LBV (C. f. v. LBV), as well as the production strain Y. enterocolitica serotype O: 9, which were used to obtain bacterial mass and extraction of nucleic acids by boiling and using a commercial kit. Product Description popup.authors Blyznetsova Hanna O. Bolotin Vitaliy I. Dragut Svitlana S. Kalinichenko Tetiana V. Marchenko Nataliia V Pikun Olena Yu. Poltoratska Hanna A Ramazanova Taisiia P popup.nrat_date 2021-02-16 Close