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Registration Number

0221U103210, 0116U000222 , R & D reports

Title

To study the immunochemical properities of recombinant analogues of chlamydia imunogenic proteines

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Head

Herilovych Anton P,

Registration Date

16-02-2021

Organization

National Scientific Center Institute of Experimental and Clinical Medicine

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A complex of bioinformatics studies on the database creation of a nucleotide sequence of genes of three species of chlamydia - C. abortus S26 / 3, C. suis MD56 and C. psittaci 6BC. According to the results of these studies, proteins CAB264, CAB649, Q499_0584 and CPSIT_0840 are characterized, which in comparison with analogues are characterized by high immunogenicity and are candidates for further studies. In order to obtain recombinant proteins of C. abortus, C. suis and C. pecorum, a set of primers was constructed that allows amplification of the corresponding region of the omp1 gene for subsequent cloning into the expression vector pTriEx-3. Three purified recombinant fusion proteins rNi-omp1-abortus, rNi-omp1-suis and rNi-omp1-pecorum with a molecular weight of 42 kDa were obtained by metal chelate chromatography at concentrations from 9 to 15 mg / l. Their activity and specificity in the study of Western blotting with specific anti-6His serum were proved. During using recombinant protein rNi-omp1-abortus as an antigen for solid-phase indirect ELISA showed false-negative results compared to the commercial analogue, with indicators of diagnostic sensitivity and specificity, as well as overall efficiency were at the level of 68.89%, 100%, 26% respectively  

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Blyznetsova Hanna O.
Bolotin Vitaliy I.
Zavhorodnii Andrii I.
Kalinichenko Tetiana V.
Obukhovska Olga V
Ramazanova Taisiia P.
Solodjankin Oleksij S.

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2021-02-16