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Information × Registration Number 0221U103213, 0116U000243 , R & D reports Title To study the biological and molegular-genetic propertties of reference and epizooticvirus strains and cell cultures for long-term storage sn conditions cryobank national collections of pathogens and cell cultures NSC "IECVM" popup.stage_title Head Stegnyi Maryna Yu., Кандидат біологічних наук Registration Date 16-02-2021 Organization National Scientific Center Institute of Experimental and Clinical Medicine popup.description2  The results of research on the study of biological, molecular genetic and karyological properties of cell cultures for long-term (29 years of RT) are presented; (15 years of RK-15); (8 years RK-15-IEKVM), storage in the cryobank of the national collection. The passport of culture of cells of a kidney of a pig is made (РК-15) and developed a methodology for long-term storage of cell cultures of calf kidney (RT) and pig kidney (RK-15; RK-15-IEKVM) in the cryobank of the National Collection of cell cultures of NSC "IEKVM". The antigen-producing biological activity of FLK-BLV transplanted culture strains (FLK-Pol; FLK50 / 100; FLK-SBBL) during long-term storage from 55.5 to 206.5 months was studied. The results of research on the study of biological properties of VCM strains for long-term up to 136 months of storage in a cryobank are presented. According to the results of research, a passport of transplanted cell culture (FLK-Pol) was drawn up; developed SOP for the method of cryopreservation of CM viruses of the first, second and third serotypes; SOP for cryopreservation and long-term storage of cell lines of virus-producing cell culture FLK-BLV. The methodology of long-term storage of VHM strains and virus-producing transplanted culture of FLK-Pol cells in the cryobank of the National Collection of NSC "IEKVM" is formed. Methodical recommendations "Cryopreservation and long-term storage of Marek's disease viruses of the first, second and third serotypes". Product Description popup.authors Isakov Magomed M Grytsyna Natalya V Yurchenko Olga M. popup.nrat_date 2021-02-16 Close