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Information × Registration Number 0221U103341, 0116U000231 , R & D reports Title Develop a system for monitoring, prediction, prevention and control of specific highly dangerous and economically important viral infections of poultry to produce biologically safe poultry products according to the standards of the European Union and the WTO popup.stage_title Head Stehnii Borys T., Доктор ветеринарних наук Registration Date 18-02-2021 Organization National Scientific Center Institute of Experimental and Clinical Medicine popup.description2  The purpose of the work is to search for new production strains of influenza viruses (subtypes H5 and H7) and Newcastle disease to replace obsolete production strains in diagnostic test systems and improve the regulations for the production of domestic reference standards. Obtaining inactivated antigens of epizootic isolate «Pivdenna Holding» of IBВ virus and obtaining positive blood sera. Research methods - virological, molecular-biological, serological. During the reporting period, comparative studies of antigenic properties of inactivated influenza virus antigens of subtypes H5N1 and H5N2 obtained in different years and from different hosts; selection of new strains of influenza A virus subtypes H5N2 (A/Teal/ Dzhankoy/4-17-11/2010) and H7N3 (mallard/AN/23-15-02/11) to improve domestic diagnostic test systems. Regulations for restoring the biological properties of highly pathogenic influenza A virus/white-fronted goose/AN/1-15-12/16 (H5N8) have been worked out. Declarative patents of Ukraine for the utility model № u201808856 «Strain of highly pathogenic avian influenza virus A/white-fronted goose/AN/1-15-12/16 (H5N8)» and № u20180836 «Inactivated antigen of influenza virus subtype H5N8 for serological diagnosis of avian influenza». Studies on the production of highly specific and highly active blood sera for the strain «Pivdenna Holding» virus of infectious bursal disease (IBD) were also carried out. Work was also carried out to restore the biological properties of the epizootic isolate «Pivdenna Holding» using primary trypsinized cultures of quail fibroblasts. During the research, the parameters of IBD virus inactivation and production of its experimental series for avian immunization were worked out. Regulations for restoring the biological properties of Newcastle disease viruses of the La-Sota production strain, isolates from synanthropic (pigeon/Donetsk/№10/26-6/08) and wild (mallard/Askania Nova/434778TM/02) birds have been worked out. Product Description popup.authors Kolesnyk Olena s Muzyka Denys V. Rula Oleksandr M. Stegniy Anton B. Tkachenko Semen V. Usova Larysa P. popup.nrat_date 2021-02-18 Close