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Information × Registration Number 0221U103627, 0120U104290 , R & D reports Title Affine sensor system based on "smart" hybrid nanocomplexes for determination of specific nucleic acid sequences". Section 1. Development and optimization of the sensory system. popup.stage_title Head Borkovska Lydmila V., Кандидат фізико-математичних наук Registration Date 23-02-2021 Organization VE Lashkarev Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine popup.description2 The objects of study - AgInS2/ZnS quantum dots and fluorescein unmodified and modified by mp-19 oligonucleotides; gold nanoparticles modified with dp-18 oligonucleotides; 80-basic oligonucleotide 80-bcr-abl. The aim of the work is to study the changes in the luminescent characteristics of quantum dots caused by their interaction with Au nanoparticles and oligonucleotides. Research methods - photoluminescence. For development of an optical affine sensor system it was necessary to study how the photoluminescence of colloidal AgInS2/ZnS quantum dots (QDs) and fluorescein changed with the addition of Au nanoparticles and oligonucleotides. It is found that conjugation of a long 80-mer oligonucleotide with a short oligonucleotide attached to the QDs results in the increase of QD luminescence intensity. It is found that both QD and fluorescein photoluminescence intensity decreases with the addition of a long 80-mer oligonucleotide conjugated with Au nanoparticles. It is shown that quenching of fluorescein luminescence intensity is the greater, the higher the concentration of the target 80-mer oligonucleotide in solution. In the case of using of the QDs of different sizes, the decrease in luminescence intensity is accompanied by a shift of luminescence band to the long-wavelength spectral region (on up to 10 nm). The obtained results show the possibility for detection in solutions of specific nucleic acid sequences using AgInS2/ZnS QDs. Prospects for future development of the objects of study - improvement of technologies for synthesis of nanocrystals based on A1A3B6 compounds with intense photoluminescence in the visible spectral range for biological applications and improvement of the method of registration of specific nucleic acid sequences using Au nanoparticles and luminescent nanocrystals based on A1A3B6 compounds. QUANTUM DOT, AgInS2/ZnS, Au NANOPARTICLE; FLUORESCEIN, PHOTOLUMINESCENCE, BIO-SENSOR Product Description popup.authors Kozoriz Kostyantyn O. Stara Tetyana R. popup.nrat_date 2021-02-23 Close