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Registration Number

0221U103668, 0118U005174 , R & D reports

Title

Development of "smart" portable signaling device for general toxicity of solutions based on enzyme multibiosensor. Section 2. Creation of bioselective elements of sensors and protocols for the determination of toxic substances

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Head

Soldatkin Oleksandr O., Доктор біологічних наук

Registration Date

23-02-2021

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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 As part of the stage, the reproducibility of signals during continuous operation and the operational stability of bioselective elements of a multibiosensor device based on AcChE, BuChE, urease, GOD were checked. It is shown that all enzyme systems of the multibiosensor are characterized by good reproducibility with an error of measurement of RSD no more than 8%, both at direct definition of substrates, and at inhibitory definition of toxic substances. The optimal storage conditions of bioselective elements based on different enzyme systems are determined and new methods of long - term storage of the multibiosensor are proposed. In addition, it was studied how the reproducibility and stability of the preparation of different enzyme membranes change depending on the used procedure of immobilization of biological material, which will significantly improve the reproducibility of the manufacture of the multibiosensor device.

Product Description

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Arkhypova Valentina M.
Kucherenko Ivan S.
Mruga Daryna O.
Soldatkin Oleksii P.
Shkotova Lyudmyla V.

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2021-02-23