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Information × Registration Number 0221U104055, 0118U005171 , R & D reports Title Affinity sensor system based on "smart" hybrid nanocomplexes for determination of specific nucleic acid sequences. Section 2. Development of "smart" hybrid nanocomplexes popup.stage_title Head Rachkov Oleksandr E., Кандидат біологічних наук Registration Date 01-03-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 The aim of stage 3 of the project is to study the interactions between different nanoobjects modified by oligonucleotides and free specific nucleic acid sequences for further development of an affinity sensory system based on "smart" hybrid nanocomplexes. Two experimental approaches have been proposed to determine the photoluminescence intensity of modified AgInS / ZnS semiconductor nanocrystals. If in the first the intensity of photoluminescence of AgInS nanocrystals is measured in samples with a volume of 100 μl in the wells of a special plate using a scanning spectrofluorimeter, in the second on an experimental setup at the V.Ye. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine measures samples with a volume of 20-25 μl, which are in glass capillary tubes. The study of changes in the photoluminescence intensity of modified AgInS / ZnS semiconductor nanocrystals when mixed with modified AuNPs and free oligonucleotides 80-bcr-abl revealed the following trend: an increase in the concentration of 80-bcr-abl in the range of 20-500 nM in the presence of AuNPs-DP leads to reducing the PL intensity of modified semiconductor nanocrystals. This result may indicate partial quenching of PL with the possible formation of triple hybrid nanocomplexes.   Product Description popup.authors Nesterenko Yevheniya A. popup.nrat_date 2021-03-01 Close