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Information × Registration Number 0221U104489, 0118U001559 , R & D reports Title The mechanisms of antibiofilm activity of adamantane derivatives popup.stage_title Head Vrinchanu Nina O, Доктор медичних наук Registration Date 26-03-2021 Organization Institute of Pharmacology and Toxicology of the National Academy of Medical Sciences of Ukraine popup.description2  Study's object adamantyl or N-alkyl radicals containing compounds with activity against bacterial biofilms. The aim of the study was investigation of adamantane derivatives antibiofilm activity general mechanisms. Complex of modern physico-chemical, biochemical, microbiological, histochemical, microscopic, molecular, pharmacological and statistical methods was used in the study. It was established for the first time that compounds with adamantyl (AM-166, KVM-97) and N-alkylaryl (KVM-194) radical disrupted the biofilms formation of S. aureus, E. coli, and P. aeruginosa, inhibited the expression of ica and icaA genes and caused genomic variability in S. aureus (revealed 3 DNA fragments in the range of 400-500 bp and variability in the range of 1000-2000 bp). The influence of compounds on biofilms was associated with changes in the components of the matrix, the ability to influence persister cells. The adamantane-containing compounds disrupted QS-dependent processes in P. aeruginosa (synthesis of pyocyanin, hemolysins, and autoinducers), and blocked efflux pumps in E. coli which was confirmed by experiments with ethidium bromide and genes tetA and tetR expression in E. coli. Product Description popup.authors Dudikova Darya M popup.nrat_date 2021-03-26 Close