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Total number
Full text
Number of local repositories
Full text
0221U104494,
0119U000437
,
To improve the way of transfection of generative cells of a pig.
Pocherniaev Kostiantin F., Доктор сільськогосподарських наук
28-03-2021
Institute of Pig Breeding and Agro-Industrial Production of the National Academy of Agrarian Sciences of Ukraine
The method of transfection of generative pig cells has been improved, which consists in the use of differential isolation of DNA from the culture medium and sperm to confirm the transfection of gametes (sperm) by circular double-stranded plasmid DNA. By amplifying the DNA of plasmid pET-28c isolated by a differential method, the penetration of plasmid DNA into sperm was proved. The specificity of DNA amplification of plasmid pET-28c isolated by differential method from sperm was proved using oligonucleotide primers (T7 promoter / T7 terminator) and obtaining the expected PCR product with a size of 314 bp Among the putative mechanisms of internalization of plasmid DNA by cells can be almost all known types of endocytosis. A double-stranded linear DNA of plasmid pET-28c was obtained, which will be used in the following research works. Preparation for publication of methodical recommendations "Method of confirmation of sperm transfection".
2021-03-28
Updated: 2025-12-08
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