Search academic texts

Information × Registration Number 0221U105088, 0119U101474 , R & D reports Title Investigation of the accumulation of recombinant and native compounds in plant systems popup.stage_title Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 04-06-2021 Organization Institute of Cell Biology and Genetic Engineering popup.description2  Vector constructs for overexpression of WUS and STM transcription factors of the great duckweed Spirodela polyrhiza were created. Three different major sequences of SpSTM-1, SpSTM-2 and SpSTM-3, were cloned into the pIHBV16 expression vector to further test their functionality and physiological effects in plants. The possibility of transient expression with a vector in which the target sequence (GFP gene) is under the control of a modified U4 promoter was also investigated, and a part of the sweet potato leaf curl virus (SPLCV) genome was added, which provides multiple copies of the target in the cytoplasm of a plant cell. The possibility of high-yielding and well-reproducible transient expression of reporter protein in the biomass of Lactuca sativa cultivars has been demonstrated, as well as the fundamental possibility of expression in Landoltia punctata duckweed. It has been shown that chitosan affects the content of dopamine in a number of plant species, but different species respond to its content in the environment differently; the reaction also depends on the concentration of the elicitor. Salinization, modeled by adding sodium chloride to the nutrient medium, causes the same reaction in different plant species - the dopamine content decreases sharply and this decrease is dose-dependent. Product Description popup.authors Belokurova Valeriia B. Kishchenko Olena М. Lystvan Kateryna V. Peterson Anton A. Rudas Volodymyr A. Sindarovska Iana R Symonenko Yurij V Shcherbak Nataliia L. popup.nrat_date 2021-06-04 Close