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Information × Registration Number 0221U105646, 0115U005179 , R & D reports Title Development of nanobiosensor technologies based on nucleic acid and nanocrystalline structures for the diagnosis of genetic and infectious diseases popup.stage_title Head Soldatkin Oleksii P., Доктор біологічних наук Registration Date 02-08-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 New biologically functionalized semiconductor nanomaterials were created using self-organization processes at two levels of the hierarchy (nanocrystalline and molecular). Nanoobjects with predictable composition, crystallinity, morphology, surface states, size, position and orientation were obtained using a number of methods for growing micro- and nanosized CdS crystals (heteroepitaxia, microtigel, vapor-crystal and vapor-liquid crystal with different catalysts). By studying the photoluminescence of semiconductor nanocrystals functionalized with thiolated oligonucleotides P2, the process of hybridization of P2 with fully complementary oligonucleotides T2 at a concentration of 4 10-7 M was registered. Piezoelectric studies using a microbalance of a quartz crystal with a bionanostructured material based on CdS nanocrystals and P2 oligonucleotides allowed registering 10-9 g of T2 oligonucleotides. When using an array of brush-type CdS nanocrystals at the first hierarchical level and thiolated mod-Ph oligonucleotides at the second level, the formation of molecular monolayers on the crystal surface was investigated by conductometry and electrochemical impedance spectrometry in the frequency range 1 Hz - 100 kHz. The created semiconductor nanocrystalline electrodes with mod-Ph layer allow to qualitatively and quantitatively detect the difference in their interaction with fully complementary oligonucleotides P1 and partially complementary oligonucleotides BCR at a concentration of 0.1 - 10 nM. Product Description popup.authors Рачков О.Е. popup.nrat_date 2021-08-02 Close