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Information × Registration Number 0221U105843, 0120U104770 , R & D reports Title Development of fluorescent diagnostic materials for hybridization assays and studies by polymerase chain reaction popup.stage_title Head Tatarets Anatolii L., Registration Date 06-09-2021 Organization State Scientific Institution Scientific and Technological Complex" Institute of Single Crystals "of the National Academy of Sciences of Ukraine popup.description2  Purpose of work – development of domestic fluorescent materials for the hybridization analysis, the PCR diagnostics of genetic and infectious diseases, in particular virus of COVID 19, the detection of genetically modified organisms (GMOs) and other nucleic acid studies. Purpose of the first stage in 2021 year – synthesis of reactive monomeric dyes-intercalators in quantities sufficient for further preparation of dimeric dyes. Obtaining of reactive probes suitable for the conjugation with the oligonucleotides. Preparation of dimeric dyes-intercalators. Comparison of properties of monomeric and dimeric dyes-intercalators under PCR conditions. Research objects – monomeric and dimeric dyes-intercalators based on derivatives of acridine orange and monomethine cyanine dyes; FLT-sensitive fluorescent dyes of different classes. Research methods – organic synthesis, spectral and other physical and photophysical research methods – NMR-spectroscopy, mass-spectrometry, electron absorption and fluorescence spectroscopy. Results obtained at the first stage of the project in 2021 year: – Methods for the synthesis of reactive monomeric dyes-intercalators based on the derivatives of acridine orange and asymmetric cyanine dyes were improved. The synthesis of monomeric dyes in quantities sufficient for further production of dimeric dyes-intercalators was carried out. – Developed and improved methods for obtaining structural analogues of commercial dyes-intercalators SYBR Green I and YOYO-1. – The synthesis and purification of reactive FLT-sensitive dyes of various classes of fluorescent compounds were carried out. – The structures of the synthesized FLT-sensitive dyes were established, and their spectral and photophysical properties were studied. – Methods for the synthesis of dimeric dyes-intercalators have been developed. – Data on the spectral and photophysical properties of the obtained dimeric dyes in solutions were obtained. Product Description popup.authors popup.nrat_date 2021-09-06 Close