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Information × Registration Number 0221U106574, 0121U114089 , R & D reports Title Development of enzyme-linked immunosorbent assays for the detection of IgG antibodies to Borrelia burgdorferi in human serum or plasma popup.stage_title Head Trokhymchuk Tetiana Yu., Кандидат біологічних наук Registration Date 14-12-2021 Organization Private Joint-Stock Company Scientific-Production Company "Diaprof-Med" popup.description2 Objective is to develop ELISA test kits for detection of anti-Borrelia burgdorferi IgG in human serum or plasma. Methods of research are ELISA, protein chromatography, microbiological, biochemical methods of bioconjugation. During the reporting part the research of test kit construction development was performed: optimal conditions for conjugates synthesis based on monoclonal antibodies and Borrelia burgdorferi antigens from prokaryotic and eukaryotic expression systems, transient transfection of mammalian cell cultures with recombinant plasmids and production of Borrelia burgdorferi antigens in cell culture, selection of optimal conditions of chromatographic purification of Borrelia burgdorferi antigens, selection of stabilizing solution for conjugate dilution, which provides their high activity during storage, selection of samples and conjugates diluents, selection of the most effective pair of biological products in composition of immunosorbent and conjugate, selection of polystyrene plate for immunosorbent, selection of viable option of test kit with the most effective pair of biological products in composition of immunosorbent and conjugate, adaption of ELISA procedure for standard ELISA equipment and ELISA analyzer. Product Description popup.authors Holubovska Anastasiia O. Horlov Andrii Yu. Zhylikhovska Tetiana V. Makarets Olena M. Pavlenko Kateryna V. Serdiuk Oleksii H. Tsybulska Lidiia I. popup.nrat_date 2021-12-14 Close