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Information × Registration Number 0221U106622, 0120U104548 , R & D reports Title Molecular components of invadopodia as prognostic factors for human malignant breast cancer popup.stage_title Head Rynditch Alla V., Доктор біологічних наук Registration Date 16-12-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 The research object – mechanism of functioning of protein TKS4 isoforms (TKS4 and TKS4b) and verprolines WIP and CR16 as invadopodia components. The research subject – protein-protein interactions, posttranslational modifications, and role of TKS4 isoforms and verprolines WIP and CR16 in cellular processes, as well as their expression in different breast cancer types. The research aim – to analyze the expression levels and functionally characterize TKS4, TKS4b, WIP and CR16 as putative biomarkers for breast cancer tumors metastatic potential assessment. Methods of research – Western blot analysis, co-immunoprecipitation, GST pull-down assay, immunofluorescence assay, transferrin internalization assay, stable cell lines generation via CRISPR/cas9 and Flp-In technologies, wound healing assay, MTT-test, total RNA extraction from breast cancer samples, RT-qPCR. TKS family of scaffold proteins and verprolines play an important role in cell invasion, particularly, promote metastasis. As a result of the project, we identified new protein partners for TKS4, WIP and CR16 among proteins associated with cell malignization; determined the peculiarities of TKS4b isoform and CR16 functioning in cellular processes and showed increased expression levels of TKS4 and WIP genes in HER2-enriched breast cancer samples, which allows to offer them as potential biomarkers of breast cancer tumors.   Product Description popup.authors Gryaznova Tetyana Anatoliіvna Gubernatorova Anastasiya O. Kropyvko Sergii V. Pankyvskyi Sergii Volodymyrovych popup.nrat_date 2021-12-16 Close