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Information × Registration Number 0221U106695, 0120U104676 , R & D reports Title Study of the state of respiratory, cardiovascular and immune systems in patients with pneumonia COVID-19 after transplantation of cryopreserved allogeneic mesenchymal stem cells popup.stage_title Head Shablii Volodymyr A., Кандидат біологічних наук Registration Date 17-12-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 The objects of study were biomolecules in the blood of patients with severe COVID-19 disease after mesenchymal stem cells transplantation. The subjects of research were microRNA, cytokines, chemokines and immune cells in blood of patients with COVID-19 after mesenchymal stem cells transplantation. The aim was to study the dynamic changes of biomarkers in the blood in patients with severe COVID-19 pneumonia after mesenchymal stem cells transplantation and to determine diagnostic markers associated with the functional state of the respiratory, cardiovascular and immune systems. Research methods included isolation of miRNA from blood plasma, cDNA synthesis on miRNA, real-time PCR, ELISA, fluorescence-activated cell sorting. Results and novelty of the current project: changes in the levels of pro- and anti-inflammatory cytokines, microRNA and different populations of immune cells were analysed in the dynamics within 28 days from the onset of the disease in COVID-19 patients with and without MSC transplantation. The study was focused on miRNA and biomarkers, which may be used as prognostic factors of the fuctional state of immune, respiratory and cardiovascular systems. Correlation analysis was performed to determine the relationship between levels of biomarkers in peripheral blood and levels of activated immune cells. The collected data can be used to predict and timely diagnose the development of possible complications during the COVID-19 progression. Product Description popup.authors Anopriyenko Olga V. Bileka Olena S Vega Aliona R. Duda Oleksandr К. Karlovskii Oleksandr A. Nikulina Viktoriia Viktorivna Rybak Mariia Yu. Skrypkina Inessa Ya. Chybisov Oleksii L Shablii Yulia M popup.nrat_date 2021-12-17 Close