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0221U106739,
0120U104574
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Combined test systems for diagnosis and analysis of innate immunity gene expression upon dangerous viral infections
Tukalo Mykhailo A., Доктор біологічних наук
18-12-2021
Institute of Molecular Biology and Genetics of NAS of Ukraine
It’s been collected the samples and created clusters of RNA from the blood specimens of healthy donors and patients with COVID-19. It’s been tested the conditions of qPCR analyses and established the level of relative expression of 10 genes of innate immunity on 59 healthy donors and 52 patients with acute form of COVID-19 in order to bring on the combined expression test system, for example, MX1, OAS1, RNASEL, EIE2AK2, IL8, IL6, TNFα, IL10, CD4, F5. It’s been found the significant difference in levels of relative expression between control group and patients with COVID-19. Statistic and bioinformatics analysis lead to the suggestion of biomarker expression sets with high levels of sensitivity, specificity, accuracy which consist of 3-8 genes and allow to diagnose the hyper inflammation, hyper coagulation, lymphopenia in the peripheral blood cells in the patients with COVID-19, and making the combined expression test system. It’s been demonstrated the highest levels of sensitivity, specificity, accuracy at the combination of three expression biomarkers: F5, MX1, CD4 (Ac.0,9; Se.0,92; Sp.0,88), meanwhile the best mark of prognostic value belongs to the set of biomarkers (OR) that consists of 6 genes: F5, MX1, RNASEL, CD4, IL10, EIF2AK2 (OR=134,37). It’s been developed the protocols for mentioned above combined PCR tests for determining of viral pathogens and expression system.
Gerashchenko Ganna V.
Gudzera Olga Yo
Kashuba Volodymyr I.
Marchyshak Tetiana V.
Melnichuk Nataliia S.
Nikolaiev Roman O.
Nuzhny Olexander O.
Tkachuk Zenoviy Y.
Tukalo Mykhaylo A.
2021-12-18
Updated: 2025-12-07
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