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Information × Registration Number 0221U106950, 0120U100197 , R & D reports Title Target gene delivery into mammalian cells using novel native biocompatible polymeric nanomaterials popup.stage_title Head Finiuk Nataliya S., Кандидат біологічних наук Registration Date 23-12-2021 Organization Institute of Cell Biology popup.description2  It was found that after immobilization of plasmid DNA with polymeric carriers F8DM1 and BP83-1, they were able to deliver plasmid DNA pEGFP-N1-p53, which encodes gene of the p53 protein, in human embryonic kidney HEK293T cells, human adenocarcinoma cervical HeLa cells, human glioblastoma U251 and U25 87 MG cells and non-small cell human lung adenocarcinoma A549 cells. Polymers BP83-1 and F8DM1 also efficiently delivered the plasmid DNA pFlag-P21WT, which encodes gene of the p21 protein, to HEK293T, HeLa, U251 and MCF-7 cells. Optimal concentrations of polymers for delivery of plasmid DNA to cells have been identified. Polymeric carriers BP83-1 and F8DM1 showed significantly higher efficiency of delivery of the P53wt gene in HEK293T and HeLa cells, compared with such efficiency of polyethyleneimine. Transfection of cells with polymexes of polyDMAEMA carriers BP83-1 and F8DM1 with pEGFP-N1-p53 or pFlag-P21WT led to a significant inhibition of cell viability of HeLa cells, which can be explained by changes in cells due to the expression of delivered genes of p53 and p21. An increase in the number of HeLa cells in the G2 phase after their transfection with the BP83-1 / pFlag-P21WT complex was detected, which indicates the blocking of these cells in the G2 / M phase of the cell cycle. The obtained results indicate a high potential of polyDMAEMA as carriers for the delivery of genes of interest to mammalian cells. Product Description popup.authors Kozak Yuliia S. popup.nrat_date 2021-12-23 Close