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Information × Registration Number 0221U107059, 0119U002361 , R & D reports Title Biocompatible polymeric materials of wound healing and antimicrobial action with immobilized proteolytic enzyme serratiopeptidase popup.stage_title Head Romanovska Iryna I., Доктор біологічних наук Registration Date 26-12-2021 Organization Physico-Chemical Institute O. Bogatsky National Academy of Sciences of Ukraine popup.description2 Objects of study: Serratiapeptidase Serratia E15, polyvinyl alcohol, sodium alginate, sodium carboxymethylcellulose, chlorhexidine bigluconate, gauze wipes. The aim is to obtain a biogel based on the sodium salt of carboxymethylcellulose and gauze wipes with immobilized in a complex polymer matrix PVA / sodium alginate serratiopeptidase, antimicrobial action, promising for wound therapy. The scheme of isolation of seratiopeptidase is developed, the homogeneous enzyme with M.m. ̴50 kDa, with high total proteolytic, fibrinolytic and collagenase activity. The batches developed are an enzyme immobilized in a carboxymethylcellulose sodium salt gel and an enzyme immobilized on gauze bandages with polyvinyl alcohol / sodium alginate. It was found that immobilization significantly improves the stability of the enzyme in a solution with a pH of 5.5, which simulates the pH of the wound contents To enhance the antimicrobial effect, it is proposed to add chlorhexidine bigluconate to immobilized drugs. Sterilization with γ-irradiation at a dose of 23 kGy gauze bandages and production of biogel under aseptic conditions are proposed. It was found that γ-irradiation of gauze bandages with immobilized seratiopeptidase at a dose of 23 kGy promotes their sterility, maintaining a high level of activity (up to 73.2%), as well as quantitative storage (3 months); proteolytic activity of sterile manufactured gel was 80%. The obtained materials have antimicrobial action against test strains of Staphylococcus aureus ATCC 25923 F-49, Candida albicans ATCC 885-653. A scheme for obtaining a new stable highly active dressing material with complex proteolytic activity, antimicrobial action has been developed. It was found that in 24 hours the efficiency of in vitro hydrolysis of excised wound scab by an enzyme attached to a gauze bandage was 1.34 times higher than that of free serotiopeptidase, due to the prolongation of the immobilized enzyme.   Product Description popup.authors Dekina Svitlana Sergiivna Ryzhak Oleksandra А. Shesterenko Yevheniya A. Shesterenko Yuliya А. popup.nrat_date 2022-03-09 Close