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Information × Registration Number 0221U107063, 0116U007522 , R & D reports Title Functional significance ITSN-containing complexes in regulation of molecular pathways of a cell popup.stage_title Head Rynditch Alla V., Доктор біологічних наук Registration Date 26-12-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 The aim of the project is to determine the functional roles of multicomponent complexes, which include scafold protein intersectin, during the formation of invadopodia, neurogenesis, and in the nucleus. Research methods - real-time PCR, mammalian cell culture and transfection, cloning, expression and purification of recombinant proteins, Western blot analysis, immunoprecipitation, fluorescence laser confocal microscopy, knockout using CRISPR spectrum, Knockdown, and RNR. A large number of proteins and their macromolecular complexes are involved in metastasis, in particular scafold proteins, which can not only serve as a platform for the collection of such complexes, but also regulate the intensity and specificity of cellular signals. Such proteins include intersectin families (ITSN1 and ITSN2), TKS (TKS4 and TKS5) and verproline (WIP, CR16 and WIRE). In this paper, we demonstrated the interaction of intersectins, TKS and verprolines with a large number of new partners, which significantly expanded the understanding of processes such as cell movement and invasion, endocytosis and vesicular traffic, nuclear cell formation and alternative splicing. The results significantly expand the existing understanding of the role of protein families ITSNs, TKSs and verprolines in the formation of invadopods, influence on gene expression, post-translational modifications, regulation of actin cytoskeleton and vesicular traffic and may be useful for developing new and predictive approaches to treatment and disease.   Product Description popup.authors Herasymchuk Dmytro O Gryaznova Tetyana Anatoliіvna Gubernatorova Anastasiya Oleksandrivna Gubar Olga S. Kozyrieva Кateryna О. Kropyvko Sergiy Viktorovych Lavrynenko Kyrylo D. Pankyvskyi Sergii Volodymyrovych Foienko Inna M. Iungin Olga S. popup.nrat_date 2022-03-09 Close