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Information × Registration Number 0221U107128, 0120U104156 , R & D reports Title Differential control of regulatory networks involved in the maintaining of epithelial-mesenchymal plasticity of breast adenocarcinoma cells popup.stage_title Head Drobot Liudmyla B., Доктор біологічних наук Registration Date 27-12-2021 Organization Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine popup.description2 Overexpression of Ruk/CIN85 in 4T1 mouse breast adenocarcinoma cells has been shown to increase mRNA expression levels of a number of key transcriptional factors that induce mesenchymal markers expression and inhibit the expression of epithelial ones At the same time, decreased expression of a number of enzymes involved in the degradation of the extracellular matrix was demonstrated, as well as divergent changes of the expression levels of the components of plasminogen activation system and dysregulation of mRNA synthesis of key components of the vitamin D3 auto/paracrine system. At the same time, down-regulation of Ruk/CIN85 led to the opposite consequences. The modulating role of Ruk/CIN85 in the control of expression of reprogramming genes and genes of markers of cancer stem cells was also established. Simultaneously, overexpression of Ruk / CIN85 in 4T1 and MCF-7 cells has been shown to significantly increase the expression/activity of key enzymes/components of glycolysis and, conversely, to reduce the activity/expression of the enzymes of oxidative phosphorylation. Moreover, in 4T1 cells with up-regulation of Ruk/CIN85 a significant increase in the levels of activity/expression of enzymes involved in extracellular matrix remodeling, control of invasive potential and chemoresistance, enzymes/molecules involved in the control of redox status were also demonstrated. For most parameters, reverse changes were observed in 4T1 cells with knockdown of Ruk/CIN85. The identified patterns of changes in the transcriptome and metabolome of breast adenocarcinoma cells for the first time showed the involvement of adaptor protein Ruk/CIN85, depending on its expression levels, in the control of dedifferentiation/differentiation processes, and EMP associated with epithelial-mesenchymal-amoeboid/amoeboid-mesenchymal- epithelial transitions.  Product Description popup.authors Gerashchenko Olga Leonydivna Horak Iryna Iryna R. Korshun Ruslan Sergiyovych Mazanova Anna Oleksandrivna Yatsenko Tetiana Andriivna popup.nrat_date 2022-03-09 Close