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Information × Registration Number 0221U107279, 0121U111970 , R & D reports Title Determination of angiogenic potential of placental mesenchymal stem cells-derived clones popup.stage_title Head Shablii Volodymyr A., Кандидат біологічних наук Registration Date 30-12-2021 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  The object of research is the culture of human placenta-derived mesenchymal stem cells (MSC) and some highly proliferative single cell-derived clones. The subject of the study is the mRNAseq profile obtained as a result of total deep RNA sequencing, human placenta-derived MSC, human umbilical vein endothelial cells. The aim of this work is to determine the angiogenic potential of individual subpopulations (single cell-derived clones) of placenta-derived MSC. Research methods - isolation and culturing of human placenta-drived MSC, isolation of single cell clones, bioinformatics analysis of RNA seq. Results and their novelty. On the basis of the first stage of research, we obtained two single cell-rerived clones from bulk placenta-derived MSC. These clones had high proliferative potential. RNAseq revealed abundance of angiogenesis-related genes in clones in compare to bulk MSC. Human umbilical vein endothelial cells were isolated, culturing and cryopreserved for further development of a model system of functional assessment of angiogenic potential of single cell-rerived clones and bulk placenta-derived MSC. Product Description popup.authors Mankovska Oksana S. popup.nrat_date 2022-03-09 Close