Search academic texts

Information × Registration Number 0222U000009, 0121U114055 , R & D reports Title Use of electrochemical biosensors and fluorescent probes for analysis and improvement of 3D bioprinted tissue structures popup.stage_title Head Tananaiko Oksana Yu., Кандидат хімічних наук Registration Date 01-01-2022 Organization Taras Shevchenko National University of Kyiv popup.description2 Objects of research - solutions, sols and hydrogels of sodium alginate with biomolecules and cells; association of ionic and nonionic surfactants with fluorescent reagent eosin H in alginate-structured medium. The purpose of the work - to develop methods for measuring the electrochemical and fluorescent characteristics of solutions, sols and hydrogels of alginate in the absence and in the presence of biomolecules and cells; development of probes based on associations of organic reagents with ionic and nonionic surfactants in alginate-structured medium for the study of fluorescent, spectrophotometric and colorimetric characteristics of biomolecules and cells immobilized in alginate gel. Research methods - cyclic voltammetry, electrochemical impedance spectroscopy, fluorescence, spectrophotometry, colorimetry. Measurements of electrochemical characteristics of solutions and gels were performed using a potentiostat from PalmSens (Netherlands) using a three-electrode cell based on printed planar electrodes (DPE); the analytical signal of the surfactant in the reaction with organic reagents in the work was evaluated by fluorescence, spectrophotometry and colorimetry. Evaluation of the colloid-chemical properties of reagent-surfactant associate solutions was performed by turbidimetry. Control of the acidity of the medium in the experiment was carried out by pH potentiometry. The electrochemical and fluorescent characteristics of alginate gels in the absence and in the presence of biomolecules and cells were studied. Electrochemical studies were performed using printed planar electrodes. Carbon, platinum or gold electrodes were working. Cyclic voltammograms of the nutrient medium for cells have been studied. It has been proved that cyclic voltammetry allows to detect electroactive compounds of nutrient solution, but after contact of cells with nutrient medium changes in the concentration of its electroactive components are not observed.  Product Description popup.authors Klovak Viktoriya О. Sasʹka Vita V. popup.nrat_date 2022-03-09 Close