Search academic texts

Information × Registration Number 0222U000261, 0119U003663 , R & D reports Title Determination of regularities of formation of antibiotic resistance of persistent clinical isolates of microorganisms and of ways of its minimization in patients with leukemia popup.stage_title Head Rybalska Alla P, Registration Date 05-01-2022 Organization State Institution "Institute of Hematology and Transfusiology NAMS Ukraine" popup.description2  The object of the study -12 patients with AL,8 people of the comparison group (patients without somatic pathology), 30 people of the control group (blood donors).The purpose of the research: to evaluate the effect of lactic acid bacteria metabolites on the immune status of patients with leukemia, to determine pathogenic potential and sensitivity of cause of the infectious-inflammatory complications to antibacterial drugs via in vitro and in vivo experiments. Research methods: microbiological, laboratory-clinical, immunocytochemical Subject of research – antibiotic resistance of 321 strains of microorganisms, 54 serum samples.The nature of resistance of 321 strains – factors of infectious-inflammatory complications in patients with leukemia, which were isolated from different biotops, was studied for 20 years (2001-2020). According to international criteria of microbial resistance, 36.1% of cultures are considered multidrug-resistant (MDR), 44.2% - extremely resistant (XDR), 5.9% - panresistant (PDR). The research demonstrates influence of probiotic starter cultures on antibiotic resistance of museum and clinical strains. Co-cultivation of microorganisms in vitro with probiotic cultures after 6,9 or 12 passages revealed an oppositional phenomenon (enhancement / suppression) of antibiotic sensitivity of the studied microorganisms to individual drugs. Probiotic consumption led to an indirect positive effect on the microflora of the nasal cavity and pharynx by reducing the number or complete elimination of opportunistic bacteria in 87.5% of patients. A decrease in the concentration of INF-γ and autoimmune antibodies to INF-α was detected after probiotic supplementation, which indicates a decrease in the infectious load and reduction of inflammation markers quantity in patients. No effect of lactic acid bacteria on IL-2 production was shown.The positive effect of the probiotics was demonstrated via described in vivo and in vitro studies. Medicine. Product Description popup.authors Kyselova Olena A Mel'nyk Olena A Nemyrovska Liudmyla M Skachkova Nadiia K popup.nrat_date 2022-03-09 Close