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Information × Registration Number 0222U000496, 0119U001418 , R & D reports Title Nanozymes and nanomediators: screening, synthesis, characterization, the use in Biosensorics and enzymatic analysis based on microbial oxidoreductases popup.stage_title Head Registration Date 11-01-2022 Organization Institute of Cell Biology popup.description2 New hybrid enzyme-nanozyme electrodes based on microbial oxidases (alcohol oxidase Ogataea polymorpha; laccase Trametes gibbosa; arginine oxidase Amanita phalloides, methylamino oxidase O. polymorpha, glucose oxidase Penicillium vitale) and CuCe NZs have been constructed. The surface of the working electrodes was also modified with a thin layer of porous gold. The bioanalytical characteristics of enzyme-nanozyme electrodes based on the artificial peroxidases of different nature and microbial oxidases have been studied. It was found that in the case of the usage of alcohol oxidase (AO) and glucose oxidase (GO), combined with NZs and porous gold, the best operational parameters of the corresponding bionanozyme electrodes were observed. The carbon materials: graphene (GF) and nanotubes (NT) were screened for their pseudo-PO activity. It was shown that GF and NT shows higher pseudo PO activity in 1.3 and 1.6 fold compared to hemin (GM). Carbon materials, as promising matrixes for the immobilization of artificial enzymes, were combined with hexacyanoferrate mimetics of PO (Fe/Cu-HCF and Fe-HCF), which lead to increase the PO activity of nanocomposites. Based on the obtained carbon-nanozyme composites (NT-Fe/Cu-HCF and NT-Fe-HCF), HCF-hemin mimetics (Cu/Fe-HCF-GM and Fe-HCF-GM) and HCF-nanozymes (Fe/Cu-HCF) an amperometric sensors for the analysis of hydrogen peroxide were constructed. Amperometric bionanosensors for glucose analysis based on GO and HCF-NT have been developed. The sensitivity of the bionanzyme electrode based on GO/Fe/Cu-HCF/NT/GE is the highest (380 A · M-1 · m-2). The screening of 5 types of potential nanomediators based on HCF-NPs (Pt-HCF, Au-HCF, Pd-HCF, Pt/Ce-HCF, Ag-HCF) and metal-hybrid NPs (CuCe, Co/Pt/Pd, Ni/Pt/Pd, Pt/Fe) was conducted. It was shown that the synthesized NPs to be promising as nanomediators, as they help to improve the electronic exchange between the surface of the working electrode and the enzyme (laccase and flavocytochrome b2. Product Description popup.authors Ivash Mariya F. Gayda Galyna Z. Gonchar Mykhailo V. Gorecha Mariya V. Demkiv Olha M. Zakalska Oksana М. Zakalskiy Andriy Ye. Prokopiv Tetiana М. Serkiz Roman Ya. Spryn Chrystyna R. Stasiuk Nataliia Ye. popup.nrat_date 2022-03-09 Close