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Information × Registration Number 0222U000565, 0120U102366 , R & D reports Title Biofunctionalized semiconductor nanocrystals for the development of impedimetric DNA sensors for early diagnostics of genetic diseases popup.stage_title Head Soldatkin Oleksii P., Доктор біологічних наук Registration Date 13-01-2022 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  asks of the stage: optimization of methods of directional formation of bioshalls on the basis of oligonucleotides on the surface of semiconductor nanocrystals of different morphology and creation of a measuring cell for impediometric research Based on the analysis of the available scientific literature on the methods of conjugation of oligonucleotides with the surface of semiconductor CdS nanocrystals, it is concluded that the most promising method is the direct binding of thiolated oligonucleotides to CdS. The constructive and technological realization of the measuring flow cell is offered. The cell is designed for single use, has a plastic body. The flow channel has a volume of up to 200 μl. The cell is equipped with two measuring electrodes, one of which is a nanostructured and biofunctionalized specific oligonucleotide. Experimental studies of CdS nanocrystalline arrays using experimental methods such as conductometry, fluorescence spectrometry, and piezoelectric microweighing were performed to identify thiol functionalization processes. The fact of functionalization is proved and the optimal conditions for CdS nanocrystals are clarified. Product Description popup.authors Grynko Dmytro A Dzyadevych Sergiy V. Nesterenko Yevheniya О. popup.nrat_date 2022-03-09 Close