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Information × Registration Number 0222U000646, 0121U111116 , R & D reports Title Influence of structure and physical properties of lipid membrane on development of viral infection popup.stage_title Head Bulavin Leonid А., Доктор фізико-математичних наук Registration Date 14-01-2022 Organization Taras Shevchenko National University of Kyiv popup.description2 Research object: structure and properties of lipid membranes Purpose of work: detection of structural features and dynamic characteristics of lipid membrane during interaction with viral pathogen Research methods: small angle neutron scattering, vibrational spectroscopy, molecular dynamics Molecular dynamics computer simulation of the effect of impurities (melatonin, cholesterol, protein molecules) on the structural properties of the model lipid membrane based on dimyristoylphosphatidylcholine (DMPC) was performed. The dependence of the structural parameters of the model lipid membrane formed by the double layer of DMPC molecules in the aqueous liquid system on the content of impurities was estimated. The effect of SARS-CoV-2 coronavirus S-protein ("thorn") on the structural parameters of the model lipid membrane on model systems based on DMPC with impurities of cholesterol or melatonin in different concentrations was studied. An experimental study of the structural features of the interaction of protein molecules with model lipid membranes by small angle neutron scattering and vibrational spectroscopy was performed. The structural parameters of the following systems were compared: aqueous DMPC solution without impurities, DMPC solution with the addition of protein molecules (RBD SARS-CoV-2), DMPC solutions with the addition of 15% and 30% melatonin or cholesterol, DMPC + 30% melatonin or cholesterol + RBD SARS-CoV-2. It is shown that an increase in the concentration of melatonin in the lipid membrane leads to a decrease in the thickness of the lipid bilayer, and an increase in the concentration of cholesterol, in contrast, leads to an increase in the thickness of the lipid bilayer regardless of the phase state of the lipid system. It was also found that the interaction of the active part of the S-protein occurs even in the absence of the ACE2 transmembrane protein in the model system, i.e. with the main parts of the membrane. Product Description popup.authors Ivanʹkov Oleksandr І. Ilyash Valentyna M. Doroshenko Iryna Yu. Nikolayenko Tymofiy Yu. Soloviov Dmytro V. popup.nrat_date 2022-03-09 Close