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Information × Registration Number 0222U000746, 0116U008923 , R & D reports Title The influence of chemical toxicants on erythrocytes, immuno system and metabolic status of a person popup.stage_title Head Haidash Ihor S, Registration Date 18-01-2022 Organization The State Institution "Lugansk State Medical University" popup.description2  Abstract Report 44 P., 1 book, 195 literature sources Relevance of the topic. Toluene causes apoptosis and functional disorder in immunocompetent cells due to its effect on the state of lipid peroxidation, the system of cyclic and adenyl nucleotides. Purpose of the study. To study in vitro the effect of toluene on the structure, metabolism and acid resistance of human red blood cells, and to develop a way to correct the resulting disorders by using a drug with an antioxidant effect “Selenium active”. Materials and methods of research. Morphological (determination of morphological fractions of red blood cells and their acid resistance). Biochemical (determination of concentrations of GPL, DC, MDA, GTNR, G-6-FDH, VGTN, camp, cGMP, ATP, ADP, amp, LDH, catalase activity and sod). Statistical methods: methods of variational statistics. Research results. Toluene in vitro causes an increase in the number of degenerative, pre – hemolytic and hemolysed forms of red blood cells, activates the processes of Pol in red blood cells, which is accompanied by an increase in the concentration of GPL, DC and MDA in hemolysates, and a decrease in the activity of the enzymes aoz-catalase, sod, GTNR, G-6-FDG, the content of VGTN; under the influence of toluene in vitro, the activity of LDH and EZ of red blood cells decreases (due to a decrease in the intracellular amount of ATP and an increase in the amount of ADP and AMP). Pretreatment of red blood cells with Selenium active reduces the negative effect of toluene on the morphological state and metabolism of red blood cells in vitro, improves their acid resistance indicators. Conclusions. 1. direct contact of toluene with red blood cells in vitro causes negative morphological and metabolic disorders in them. The negative effect of toluene on red blood cells increases with an increase in the active concentration of toluene. 2.pretreatment of red blood cells with Selenium active reduces the negative effect of toluene on the morphologic Product Description popup.authors Burtsev Oleksiy V Haydash Iryna Anatolyivna Haydash Olena Ihorivna Yanchevsky Oleksandr Valeriyovich popup.nrat_date 2022-03-09 Close