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Information × Registration Number 0222U000780, 0120U101631 , R & D reports Title Development of new methods for construction of strains of the yeast Ogataea polymorpha with improved alcoholic fermentation of pentoses popup.stage_title Head Sibirny Andriy A., Доктор біологічних наук Registration Date 18-01-2022 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2  The insertional mutagenesis was performed for identification of genes disruption of which resulted in increased ethanol production from xylose during high temperature alcoholic fermentation. For this purpose several variants of insertional cassette were constructed and introduced into the genome of O. polymorpha recipient strain. The selection of transformants with increased colony size was performed on the minimal medium with 15% L-arabinose. The ability to produce ethanol from xylose and L-arabinose was evaluated in selected transformants. As a result strains with increased ethanol production from xylose and L-arabinose were obtained. Using standard molecular techniques the insertional cassette along with flanked regions was isolated from genomes of selected strains with increased ethanol production. The identification of disrupted genes involved in fermentation of xylose in O. polymorpha will be performed by sequence analysis of isolated DNA fragments. Product Description popup.authors Vasylyshyn Roksolana V Dmytruk Kostiantyn V Kurylenko Olena O. popup.nrat_date 2022-03-09 Close