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Information × Registration Number 0222U000846, 0120U101644 , R & D reports Title Pharmacological modulation of glutathione-dependent regulation and implementation mechanisms of endogenous neuroprotection and neuroplasticity - a new perspective on neuroprotection in ischemic strokes popup.stage_title Head Byelenichev Ihor F., Доктор біологічних наук Registration Date 19-01-2022 Organization Zaporizhzhya State Medical University popup.description2  The object of study is HSP70/GSH-dependent mechanisms of endogenous neuroprotection in cerebral ischemia. The purpose of the study – an experimental substantiation of administration of modulators of the glutathione system (glutathione, glutaredoxine and selenium derivatives: Se-glutathione, Se-methionine, Se-cysteine), as well as heat shock factor 1 (HSF-1) in the treatment of stroke based on experimental data revealing the role of glutathione systems in HSP70-mediated mechanisms of regulation of endogenous neuroprotection. Research methods – molecular, morphometric, biochemical, histochemical, polymerase chain reaction and immunoblotting. The results of the Scientific research work and its novelty. The action of cytotoxic agents (glutamate, hydrogen peroxide, dinitrosyl-iron complex with cysteine) on changes in the glutathione system (glutathione reduced, glutathione oxidized, the levels of thiol groups, the activity of glutathione reductase, glutathione peroxidase, and glutathione transferase), and levels of HSP protein expression in vitro as well as correlation between the parameters of glutathione system, HSP70 levels and cell death processes in vitro were studied for the first time. Parameters of endogenous neuroprotection after its initiation were studied in vitro. The role of glutathione system in HSP70-mediated regulatory mechanisms of endogenous neuroprotection was studied after modeling cerebral ischemia in rats. Based on the obtained experimental data revealing the role of the glutathione system in HSP70-- mechanisms of regulation of endogenous neuroprotection, the administration of modulators of the glutathione system modulators (glutathione, glutaredoxine and selenium derivatives: Se-glutathione, Se-methionine, Se-cysteine) as well as heat shock factor 1 (HSF-1) was experimentally substantiated for stroke treatment. Product Description popup.authors Yehorov Artem A. Aliieva Olena H. Bila Yuliia V. Bak Pavlo H. Zidrashko Halyna A. Zavhorodnia Mariia Yu. Korostina Krystyna I. Nosach Svitlana H. Parkhomenko Daria P. Popazova Olena O. Ryzhenko Viktor P. Samura Iryna B. Tykhonovskyi Oleksandr V. popup.nrat_date 2022-03-09 Close