Search academic texts

Information × Registration Number 0222U000932, 0120U102209 , R & D reports Title Interaction of operated nanobiocomposite with model systems of different degree of sensitivity and aggressiveness of the cancer process (№ 55/20-Н) popup.stage_title Head Chekhun Vasyl Fedorovych, Доктор медичних наук Registration Date 21-01-2022 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2 The laboratory regulations for the cultivation of Bacillus subtilis B-7724 have been improved due to deep aeration with simultaneous permanent destruction of the foam layer on the surface of the culture fluid. This approach optimizes the growth of the producer strain, and accordingly significantly reduces the time required to obtain the final product. In the in vitro system we investigated the peculiarities of the interaction of controlled nanobiocomposite containing nanoparticles of ferromagnet and lectin B. subtilis IMB B-7724 with model systems of different degrees of malignancy and sensitivity to antitumor drugs. In assessing the viability and proliferation of cells of the most common hormone-dependent malignancies, it was found that the most sensitive to cytotoxic effects of nanobiocomposite are human breast cancer cells with the phenotype of drug resistance to doxorubicin MCF-7/Dox, to cisplatin MCF-7/СР and receptor-negative cells of the high malignancy degree MDA-MB-231. In human prostate cancer cells, the greatest antiproliferative effect of the nanobiocomposite was observed against cells DU-145 with a high malignancy degree, which are characterized by a medium sensitivity to doxorubicin and high to cisplatin. The least sensitive to the action of the nanobiocomposite were low-malignant LNCaP cells resistant to cisplatin, as well as high-malignant PC-3 cells resistant to doxorubicin. Product Description popup.authors Didenko Gennadii V. Lykhova Oleksandra O. Lukianova Nataliia Yu. Cheremshenko Nadiia L. popup.nrat_date 2022-03-09 Close