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Information × Registration Number 0222U001184, 0118U005467 , R & D reports Title Expression of the transcription factor NF-kB and the enzymes of polyamines metabolism, the levels of polyamines and the activity of RNAses in prostate tumors as potential criteria for predicting the course of the disease popup.stage_title Head Zaletok Sophia P., Доктор біологічних наук Registration Date 24-01-2022 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description2 The expression of proteins of polyamine (PA) metabolism enzymes – arginase (Arg1), ornithine decarboxylase (ODC), spermine synthase (SpmSy), spermine /spermidine acetyltransferase (SSAT), polyamine oxidase (PAO), levels of PA, and activity of RNAase in postoperative samples of human prostate cancer were studied. It is established that with the increase of PCa aggressiveness the levels of expression of enzymes of PA synthesis – ODC and SpmSy – decrease and the level of expression of enzyme of PA catabolism (SSAT) increases. It was found that the levels of all fractions of PA in PCa, except N1-acetylspermidine and N1, N12-diacetylspermine are much lower than their values in BPH cells. For the first time, there has been an inverse relationship between spermine levels and the aggressiveness of PCa. For the first time, the highest levels of spermine, spermidine, and putrescine were found in low-risk tumors, and the lowest levels were for tumors with a high risk of progression. The strongest inverse relationship between risk groups (low → medium → high) and PA levels was found for spermine. For the first time, the existence of an inverse correlation between the levels of PA in PCa and the spread of the disease (localized / locally advanced PCa) was proved. It has been shown that high expression of p65 (NF-κB) in PCa cells is associated with an increasing stage of the disease. A progressive decrease in RNase activity with the spread of PCa was found. The results suggest that the expression of SpmSy, SSAT, spermine level, and RNase activity may serve as additional criteria for precise diagnosis, assessment of the aggressiveness of PCa, and prognosis of the disease. Product Description popup.authors Bentrad Veronika V. Klenov Oleg O. Prylutskyi Maksim Petrovich Yanish Yurii V. popup.nrat_date 2022-03-09 Close