Search academic texts

Information × Registration Number 0222U001238, 0121U112504 , R & D reports Title To study the effect of RM drugs on the metabolic activity of the microbiota and the state of the intestinal barrier in various pathological conditions and to analyze morphological and microbiological changes in patients with IBS / VC from contaminated and agricultural regions. popup.stage_title Head Tolstanova Hanna М., Доктор біологічних наук Registration Date 25-01-2022 Organization Taras Shevchenko National University of Kyiv popup.description2 The object of research is the intestinal-brain interaction. The aim of the study was to investigate the effect of oral administration of smoke nanoparticles of natural origin from the combustion of poplar wood on the course of LRS-induced neuroinflammation, glutamate / GABA neurotransmission and the state of the intestinal barrier of rats. Research methods - monitoring of clinical signs of white nonlinear rats under conditions of LRS-induced neuroinflammation (body weight, diarrhea), signs of impaired metabolic activity of microbiota (caecum mass), systemic inflammation (thymus, spleen mass); determination of paracellular permeability of the epithelial barrier by the permeability of the marker FITC-dextran, 4 kDa; registration of short-circuit current in the conditions of voltage fixation at 0 mV (µA / cm2); transepithelial resistance in response to a current pulse of 10 μA (Ohm * cm2); determination of the accumulation of L- [14C] glutamate and [3H] GABA synaptosomes; statistical analysis of the obtained results. Seven-day oral administration of the drug RM smoke obtained from the combustion of poplar wood at a dose of 180 μg / 100 g in rats with LRS-induced neuroinflammation: (i) led to a probable weight loss of animals; (ii) there was a tendency to increase the mass of the thymus (iii) did not cause significant changes in the number of destroyed dopaminergic neurons in rats with LRS-induced neuroinflammation, as determined by apomorphine test. However, the drug RM smoke probably reduced the transporter-mediated accumulation of L- [14C] glutamate and [3H] GABA in the nerve terminals of the rat brain; (iv) changes in neurotransmitter transport have been associated with exacerbations of proinflammatory changes in the intestinal barrier state under the influence of RM smoke.  Product Description popup.authors popup.nrat_date 2022-03-09 Close