Search academic texts

Information × Registration Number 0222U001314, 0119U000114 , R & D reports Title To develope the methods of correction of brain cortical and subcortical structures after craniocerebral traumas with stem-cell and immune factors in the experiment popup.stage_title Head Lisyanyy Nikolay Ivanovich, д.мед.н.Pedachenko Yevhenii Н., Доктор медичних наук Registration Date 26-01-2022 Organization The State Institution Romodanov Neurosurgery Institute National Academy of Medical Sciences of Ukraine popup.description2  The object of study - systemic and intracerebral immune responses, apoptosis, proliferation of brain cells, behavioral responses of rats with trauma in the correction of trophic factors of stem cells. The aim of the study was to investigate the possibility of correcting the effects of trauma by humoral factors produced by different types of stem cells based on the study of changes in systemic and local immunity, the development of apoptosis and proliferation of nerve cells and behavioral responses in rats at different times after trauma. Research methods - experimental, immunological, immunohistobiological, tissue culture, light microscopy. In experimental TBI in rats there are changes in the innate and acquired parts of the immune system, there are significant and long-term changes in the activity of microglial, macrophage, lymphoid immune cells contained in the brain and spleen. The use of the immunotropic drug galavit for the correction of immune changes caused a temporary inhibition of the increased activity of immune cells in rats with trauma. Trophic factors of stem cells obtained from the brain of embryos and adipose tissue by short-term 48-hour cultivation inhibited the activity of microglial and lymphoid cells, reduced cerebral edema and the number of P53 + apoptotic cells in the cortex and hippocampus of the brain, which showed normalized behavior. on the 1st, 5th, 10th day after TBI. The activity of trophic factors obtained in the cultivation of stem cells depends on the source of stem cells and methods of obtaining them. A model of TBI on nerve cell culture has been developed and the possibility of studying regeneration processes under the action of stem cells and their factors has been shown. The obtained results indicate the possibility of obtaining trophic factors of stem cells, new drugs for treatment. Product Description popup.authors Egorova Diana M Belska Lyudmyla M Velichko Olga M Gridina Nina Ya Klyuchnikova Antonina I Lisyanyi Mykola I Liubych Larysa D Palamaryova Anastasia V Pedachenko Yevhenii Н. Stajno Larisa P Stanetska Diana M popup.nrat_date 2022-03-09 Close