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Information × Registration Number 0222U001798, 0118U005171 , R & D reports Title Affinity sensor system based on "smart" hybrid nanocomplexes for determination of specific nucleic acid sequences. Section 2. Development of "smart" hybrid nanocomplexes popup.stage_title Head Rachkov Oleksandr E., Кандидат біологічних наук Registration Date 03-02-2022 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  The photoluminescence of AgInS nanocrystals was measured in 100 μl samples in the wells of a special plate using a scanning spectrofluorimeter. The effect of different surface densities of immobilized oligonucleotides with the addition of different concentrations of gold nanoparticles on the optical characteristics of quantum dots modified with a short oligonucleotide, as well as in the presence of free complementary oligonucleotides 80-brc-abl. The obtained data showed that the increase in the surface density of short oligonucleotides on the surface of quantum dots affects two preparations of quantum dots in different ways: in some there is an increase in photoluminescence, and in others there are no significant changes. Increasing the concentration of gold nanoparticles in samples with quantum dots reduces the photoluminescence both in the presence of 80-brc-abl samples and in its absence. Electrolytic aggregation of modified gold nanoparticles in samples with modified quantum dots and complementary oligonucleotide 80-brc-abl was also investigated. It was found that the presence of a long oligonucleotide in the samples does not affect the aggregation of gold nanoparticles. Product Description popup.authors Dzyadevych Sergiy V. Kalinichenko Larysa O. Nesterenko Yevheniya О. Pichiyan Nataliya V Soldatkin Oleksii P. Yarynka Dariia V. popup.nrat_date 2022-03-09 Close