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Information × Registration Number 0222U002376, 0120U104290 , R & D reports Title Affine sensor system based on "smart" hybrid nanocomplexes for determination of specific nucleic acid sequences". Section 1. Development and optimization of the sensory system. popup.stage_title Head Borkovska Lydmila V., Кандидат фізико-математичних наук Registration Date 11-02-2022 Organization VE Lashkarev Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine popup.description2 The object of study - unmodified and modified by mp-19 oligonucleotides quantum dots based on AgInS2/ZnS (MP-QDs); Au nanoparticles modified by dp-18 oligonucleotides (DP-Au); 80-basic target oligonucleotide 80-bcr-abl (80-mer). The aim of the work is to study the luminescent characteristics of QDs in their interaction with Au nanoparticles and oligonucleotides. Research methods – photoluminescence (PL). The influence on the QD PL of the concentration of nanoobjects (mp-19 and DP-Au) and incubation time of the solutions containing MP-QDs, DP-Au and 80-mer was studied. It is found that immobilization of mp-19 on the QD surface promotes the increase of the PL intensity and spectral shift of the PL band. The magnitude of the effect depends on type of QD stabilizing ligand (glutathione and mercaptoacetic acid), it increases as the concentration of the mp-19 increases. The adding of DP-Au to the QD buffer solution in the ratio of concentrations of DP-Au to MP-QDs as 0,04:1, 0,08:1 and 0,2:1 results in the decrease of PL intensity and the shift of PL band maximum. The magnitude of spectral changes increases with the increase of DP-Au concentration and incubation time of nanoobjects in solution (for tens of days). The largest changes (the decrease of PL intensity in 100 times and spectral shift of 140 meV) are observed for the DP-Au solution and glutathione-stabilized QDs in the ratio of 0,2:1. It is shown that the adding of 80-mer to the solution of MP-QDs and DP-Au increases the degree of PL quenching. The obtained result shows the possibility for detection in solutions of specific nucleic acid sequences using AgInS2/ZnS QDs and Au nanoparticles. Prospects for future development of the objects of study - elucidation of the mechanism of the effect of Au nanoparticles on the PL of AgInS2/ZnS QDs is necessary for improvement of the method of registration of specific nucleic acid sequences using QDs. QUANTUM DOTS, AgInS2/ZnS, Au NANOPARTICLE; PHOTOLUMINESCENCE, BIO-SENSOR Product Description popup.authors Kozoriz Kostyantyn O Stara Tetyana R popup.nrat_date 2022-03-09 Close