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Information × Registration Number 0222U002884, 0120U105172 , R & D reports Title Functionalization of metal SERS-substrates with biomolecules popup.stage_title Head Pirko Yaroslav V., Доктор біологічних наук Registration Date 16-02-2022 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 During the research work we suggested new approaches to functionalization of nanostructured (gold nanoparticles) surfaces of SERS-substrates based on the morphology of "inverted pyramids" to identify different types of biomolecules. In particular, the SERS substrates were functionalized using specific monoclonal antibodies for rapid and efficient detection of bacteria. Characteristic bands of E. coli and P. aeruginosa antibodies have been identified, as well as bands by which they are distinguished, which may obviously be due to different amino acids in the composition of these antibodies. To understand the mechanisms of antibody enhancement and adsorption, a study was conducted to explore the adsorption features of amino acids (such as lysine and histidine), as well as salicylic acid on a modified SERS substrate. Also, conditions were established and surface functionalization of nanostructured gold SERS substrates was developed using specific aptamers (for example, the use of aptamers against E. coli and zearalenone) as an effective platform for detecting bacteria and mycotoxins in samples. Product Description popup.authors Buziashvili Anastasiya Yu. Pirko Yaroslav V. Plokhovska Svitlana H. popup.nrat_date 2022-03-09 Close