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Information × Registration Number 0222U003384, 0119U100307 , R & D reports Title Molecular and cellular mechanisms of TRP canalopathy. It is the same alloy with NKM composition. popup.stage_title Head Zholos Oleksandr V., Доктор біологічних наук Registration Date 17-03-2022 Organization Taras Shevchenko National University of Kyiv popup.description2 Object of study: expression, mechanisms of activation and functions of receptor-directed TRPC4 channels, mechano-, osmo- and heat-sensitive TRPV4 channels and cold and menthol receptors TRPM8 in smooth muscle, skeletal muscle and epithelial cells in normal and pathogenic genesis. Subject of research: interrelation of key links of TRP-mediated signaling in interaction with other potential-dependent channels, in particular Ca2 + -activated K + channels of high conductivity (maxi-K, BKCa) and potential-directed Ca2 + channels of L-type (Ca-L) in regulation vascular tone, contractile and secretory function of the intestine and maintenance of the barrier function of the epithelium and endothelium. To study the effect of new pharmacological modulators (Pico-145, C60 fullerenes, carbon nanotubes) on TRPC4 channels and BKCa high conductivity potassium channels, which will contribute to the possible practical application of these substances for pharmacological correction of visceral and vascular smooth muscle disorders. Objective: to determine the expression and function of sensory and receptor-controlled TRP channels, as well as their interaction with other potentially dependent channels (including BKCa, L-Ca), as potential target molecules for the correction of canalopathy in hypertension of various origins, early nonmotor manifestations in Parkinson's disease (CP) and epithelial and endothelial barrier disorders. Investigate the latest modulators for muscarinic cationic current (MICat), which is mainly mediated by TRPC4 channels and, namely, highly selective Pico-145 and carbon nanotubes, as well as C60 fullerenes on BKCa high-conductivity potassium channels. Research methods: electrophysiological (patch terminal registration transmembrane ion currents and membrane potential), calciummetry using laser confocal microscopy, strain gauge, balonography, immunohistochemical identification of dopaminergic neurons, etc. (specified in the Terms of Reference).  Product Description popup.authors Akulenko Iryna V. Gorbach Oleksandr I. Dovbynchuk Taisa V. Dryn Darіya O. Kovalchuk Oleksandr I. Kot Tetyana G. Melnyk Maria I. Moroz Olesya F. Nozdrenko Dmytro M. Nuryshchenko Nataliia Ye. Prylutskyi Yurii I. Raksha Nataliya G. Svyetlova Nataliya B. Soroka Vasyl M. Stetskaya Victoriya O. Tolstanova Hanna М. popup.nrat_date 2022-03-17 Close