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Information × Registration Number 0222U003552, 0119U101474 , R & D reports Title Investigation of the accumulation of recombinant and native compounds in plant systems popup.stage_title Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 20-04-2022 Organization Institute of Cell Biology and Genetic Engineering popup.description2  For the first time the fundamental possibility of using an edible plant species - basil (Ocimum basilicum) as a producer of recombinant proteins by the method of transient expression was showed. It was proved that the accumulation of the target recombinant protein is significantly higher when genetic vectors with elements of the genomes of phytopathogenic viruses were used. Such a high accumulation of recombinant target proteins (10-20% of the total protein content) is not usually observed in edible plant species, so basil may be an alternative to inedible recombinant protein-producing species such as tobacco. During the project, a number of genetic vectors were created to optimize the accumulation of recombinant proteins in plants. During the project, vector constructs for overexpress the transcription factors WUS and STM of the great duckweed Spirodela polyrhiza were created. Three different major sequences, SpSTM-1, SpSTM-2 and SpSTM-3, were cloned into the expression vector and tested for their functionality and physiological effects in plants. As a result of the work, genetic constructs which proved the positive effect of viral suppressor of silencing of P19 genes and 5'UTR RbcS2B sequence on accumulation of recombinant GFP protein via transient expression in Nicotiana rustica L. plants also were created. Transplastom tobacco plants that synthesize recombinant antiviral protein interferon α2b were obtained and analyzed. The presence of antiviral activity of transplastomic plant extracts was confirmed in the model in vitro system - a culture of monkey cells infected with vesicular stomatitis virus. Transgenic plants with the gene of sweet protein - thaumatin II for a number of members of the legume family (alfalfa, peas, peanuts), as well as two commercial varieties of lettuce were also obtained. Product Description popup.authors Vasylenko Maxim Yu. Kishchenko Olena М. Lystvan Kateryna V. Nifantova Svitlana M. Ovcharenko Olga O Peterson Anton A. Rudas Volodymyr A. Sindarovska Iana R Symonenko Yurij V. Shcherbаk Nаtаliia L. popup.nrat_date 2022-04-20 Close