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Information × Registration Number 0222U003663, 0117U002589 , R & D reports Title The investigation of heterologous genes functioning and their impact on the adaptive characteristics of plant systems in conditions of biotic and abiotic stresses popup.stage_title Head Кучук Микола Вікторович, Registration Date 11-05-2022 Organization Institute of Cell Biology and Genetic Engineering popup.description2  Transgenic potato plants (Solanum tuberosum) and petunias (Petunia hybrids) in which extracellular ribonuclease genes (bov and ZRNase II) are expressed were obtained. The analysis of transgenic plants confirmed the increased level of extracellular ribonuclease activity, which resulted in a decrease of virus antigens accumulation and increased resistance of the obtained transgenic plants to various RNA-containing viruses. Transgenic plants of spelt (Triticum spelta L.) that contained sequences for genome editing using the CRISPR-Cas9 system were also obtained. These plants could be used to produce the plant lines with the edited genome with such agriculturally valuable traits as dwarfism (which prevents lying down of this crop in bad weather) and herbicide resistance. Transgenic tobacco and potato plants (S. tuberosum) in which cyanobacterial desaturase gene expression is also was obtained and analyzed for resistance to various abiotic stressors. It has been confirmed that the expression of cyanobacterial desaturase genes in transgenic plants increases their resistance to low temperatures and osmotic stress. A study of stress response of transgenic cultures obtained by Agrobacterium rhizogenes-mediated genetic transformation of species such as althaea (Althaea officinalis), foxglove (Digitalis purpurea L), wormwood (Artemisia tilesii). It was determined that transgenic cultures of these medicinal plants showed better adaptive characteristics and greater resistance to stress factors compared to non-transgenic control. It was also shown that the level of accumulation of Cr6 +, Ni2 + and Pb2 + ions by cultures of transgenic roots of tricolor birch (Convolvulus tricolor) is similar (or even higher) to the level of accumulation of these metals by known hyperaccumulator plants. Culture of transgenic roots accumulates significantly more heavy metals than non-transgenic roots of intact plants. Product Description popup.authors Duplij Volodymyr P Kyrpa Tetiana M Lystvan Kateryna V. Luchakivska Yuliya S. Lyoshyna Lyidmyla H Matvieieva Nadiya A. Nifantova Svitlana M. Ovcharenko Olga O Shcherbаk Nаtаliia L. popup.nrat_date 2022-05-11 Close