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Information × Registration Number 0222U003685, 0121U112332 , R & D reports Title Isolation and characteristics of actinobacteria from rhizosphere of Colobanthus quitensis (port Charcot, Booth Islands) popup.stage_title Head Gromyko Oleksandr M., Кандидат біологічних наук Registration Date 23-05-2022 Organization Ivan Franko National University of Lviv popup.description2  21 isolates of actinobacteria were isolated from samples of the rhizosphere of C. quitensis (Sharko port, Booth Island). Strains capable of growing at a temperature from 4 ºС to 45 ºС, at pH of the medium from pH 4–12 were found. Only a third of the isolates were able to grow at elevated NaCl concentrations (2.5%). In contrast, most of them were resistant to salinity with sodium sulfate (2.5 - 5.0%). Strains capable of inhibiting the growth of Mycobacterium smegmatis and multidrug-resistant strain Candida albicans have been isolated. A number of strains combined both antibacterial and antifungal activities. A significant number of isolates were characterized by their sensitivity to beta-lactam antibiotics, which may be related to cell wall structure. Prosthetic and amylolytic activity was detected in 95.8% of isolates. Slightly more than 70% decomposed carboxymethylcellulose, almost 42% of isolates were capable of nitrate reduction, and 25% showed lipolytic activity. 21.7 and 8.3% of strains showed pectinolytic and lecitase activity, respectively. Laccases and oxidoreductases did not produce the studied isolates. Most isolates produced indoleacetic acid at a concentration of 25.0 to 65.0 μg / ml. Two isolates showed an increased level of synthesis for both actinobacteria - 105.3 (Cq 72-4) and 220.7 (Cq 72-18) μg / ml, respectively. One third of the isolates solubilized zinc compounds and 4.2% were able to solubilize phosphorus compounds. The production of siderophores was not found in any of the studied isolates. The properties and phylogenetic characteristics of the isolate with a wide range of biological activities are described. The strain is classified into the genus Streptomyces. Product Description popup.authors Bilyk Bohdan Romanovych Leshchynska Oksana Mychailivna Roman Ivan Ivanovych Syrvatka Vasyl Yaroslavovych Fedorenko Victor Oleksandrovych popup.nrat_date 2022-05-23 Close