Search academic texts

Information × Registration Number 0222U004476, 0120U103770 , R & D reports Title Biotechnology development for plants of the genus Triticum to increase their yield popup.stage_title Head Morgun Bogdan V., Кандидат біологічних наук Registration Date 12-08-2022 Organization Institute of Cell Biology and Genetic Engineering popup.description2 Cell lines and plants of the genus Triticum (wheat and spelt) are the object of research. The purpose of the work is to choose the conditions for the regeneration of spelt and wheat plants from mature and immature embryos, to select the most promising genotypes with high regeneration capacity. In the course of research, experiments were conducted using equipment of domestic and foreign production. The following set of works was carried out: the minimum selective concentration of the antibiotic hygromycin B (30 mg/l) was established, which completely suppresses the regeneration of shoots in soft wheat of Podolyanka and Yatran 60 varieties; the optimal selective concentration of the herbicide phosphinothricin (5 mg/l) was established for the selection of transgenic spelt plants of the Europa variety and breeding lines No. 845, No. 853. The most promising genotypes of spelt with a high regenerative capacity were selected: using the culture of apical meristems of spelt shoots, the most promising for obtaining transgenic plants is breeding line No. 853; for the use of calli from immature embryos - breeding lines No. 2250 and No. 2273; with the use of mature embryos, breeding line No. 2273 had the highest regeneration potential. Nutrient media were selected for Agrobacterium-mediated transformation of spelt: to stimulate callusogenesis, regeneration, and rooting of regenerants. Product Description popup.authors Bannykova Mariia O Nitovska Iryna O. popup.nrat_date 2022-08-12 Close