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Information × Registration Number 0222U004493, 0118U003663 , R & D reports Title Study of molecular biological and phenotypic manifestation of transmitted genes performance and their inheritance in biotechnological plants popup.stage_title Head Morgun Bogdan V., Кандидат біологічних наук Registration Date 19-08-2022 Organization Institute of Cell Biology and Genetic Engineering popup.description2 The purpose of the work was to study the functioning of stress resistance genes, the possibility of transferring foreign genes of various origins into economically valuable plants, clarifying the peculiarities of their expression and inheritance, studying the physiological-biochemical and molecular-biological manifestation of traits determined by transgenes in biotechnological plants; elucidation of allelic polymorphism of genetic sequences involved in response to stress factors. The inheritance of the nptII gene in the T3 generation of wheat obtained as a result of self-pollination of T2 plants carrying the nptII transgene was studied - fewer transgenic plants were found than expected. As a result of studying the influence of promoter sequences on the expression level of the uidA gene in transgenic tobacco (a dicotyledonous plant), it was established that the 35S RNA promoter of the cauliflower mosaic virus is 100 times more "powerful" than the corn ubiquitin gene promoter (a monocotyledonous plant). Western European and Eastern European strains of the yeast Saccharomyces paradoxus have different sets of polymorphic variants of the microsatellite loci UBA1, YIL130 and ZEO1. The most polymorphic microsatellite locus in Saccharomyces paradoxus is the UBA1 locus. In Saccharomyces paradoxus yeast strains collected in the "red" forest, the microsatellite loci UBA1, YIL130, and ZEO1 had a longer length compared to strains that were collected in places with lower levels of radiation pollution. It was investigated that the foreign chloroplast genome has a positive effect on the growth rate of the cybrid plant compared to the parent (nucleus donor). In the parent plants of cybrids - rape varieties Westar and Pactol, Lesquerella fendleri and Orychophragmus violaceus - when grown under stressful conditions, a decrease in the amount of soluble protein and a significant increase in the increase in raw mass were found. However, they were more stress-tolerant. Product Description popup.authors Bannikova Maria О. Nitovska Iryna O. Pronina Olha V. popup.nrat_date 2022-08-19 Close