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Information × Registration Number 0222U004773, 0117U000657 , R & D reports Title Investigation of the mechanisms of tumor growth inhibition through limiting of carbohydrate supply, inhibition of glycolysis and gluconeogenesis and induction of arginine starvation popup.stage_title Head Sybirnyi Andrii A., Registration Date 27-10-2022 Organization Institute of Cell Biology of the National Academy of Sciences of Ukraine popup.description2  The proposed project was focused on dvelopment of novel approaches of cancer combination therapy based on inhibition of energy metabolism in tumor cells in combination with deficiency of certain amino acids, in particular arginine. It was shown for the first time that the combination of a low-carbohydrate diet and metformin can effectively inhibit the growth of solid tumors in mice, including highly metastatic melanoma B16. This effect was accompanied by zero mortality of tumor-bearing mice even on the 100th day after the start of treatment, and complete normalization of the functions of hematopoietic and immune systems in experimental animals. However, for the complete elimination of the tumor, a minimum dose of the traditional anticancer drug should be additionally used. It was found that the glycolysis inhibitors 3-bromopyruvate and sodium hydroxycitrate do not show a therapeutic effect towards murine NK/Ly lymphoma model, and the combination of 3-bromopyruvate with a low-carbohydrate diet generally showed an antagonistic effect. It was also established that the modulation of energy metabolism against the background of long-term starvation for arginine does not lead to an additive effect in inhibiting the growth of tumor cells. The combination of a low-carbohydrate diet, metformin, and glibenclamide together with low doses of anticancer drugs (in particular, doxorubicin) showed a pronounced synergistic effect and led to a 4-fold extension of life in tumor-bearing animals with NK/Ly lymphoma and L1210 leukemia. The obtained results indicate significant prospects for the further application of the proposed scheme of treatment of cancer patients in clinical practice. Product Description popup.authors Ivash Mariya F. Demkiv Olha M. Dmytruk Kostiantyn Vasylovych Kashchak Nataliya I. Kozak Yuliia S. Panchuk Rostyslav Ruslanovych Prokopiv Tetyana M. Sybirnyi Andrii А. Skorokhyd Nadiya R. Fayura Lyubov R. Tsyrulnyk Andriy O. popup.nrat_date 2022-10-27 Close