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Information × Registration Number 0222U005215, 0120U101972 , R & D reports Title Composite nerve conduits on the basis of polymer nanofibrils and electro-conducting materials for treatment of critical neuronal injuries popup.stage_title Head Kyrylenko Sergiy D., Кандидат біологічних наук Registration Date 25-12-2022 Organization Sumy State University popup.description2  Conducted studies on the identification of parameters for obtaining tissue-engineered structures for the construction of nerve conduits revealed that the optimal type of cells for obtaining structures are mesenchymal stem cells obtained from the human umbilical cord. The optimal seeding density of mesenchymal stem cells is 10,000 cells per square centimeter of the matrix surface. The optimal concentration of the growth factor FGF2 is 4-10 ng/ml. The optimal composition of the medium should be based on the nutrient mixture DMEM-F12 with the addition of 10% fetal calf serum and antibiotics/antimycotics in standard concentrations. Incorporated silver nanoparticles inhibit the growth of bacteria on nanomatrices at 5 μg/ml and inhibit the formation of bacterial biofilms at concentrations of 10 μg/ml. Research on the identification of parameters for the optimal colonization of modified polymer matrices by cells in in-vitro cell culture conditions allowed to optimize the construction processes of modified polymer matrices with electrical conductivity, which is necessary for the construction of nerve conduits and cardiac patches. It was established that the optimal growth of human umbilical cord mesenchymal stem cells occurs on polymer matrices, on which 2-3 layers of Ti3C2Tx mexenes were immobilized. Optimizing cell growth occurs due to nanostructural defects in the polymer-Mxene structure, detected using the latest method of positron annihilation spectroscopy Product Description popup.authors Bezkorovaina Nadiia I. Viter Roman V. Varava Yuliia V. Kazban Viktoriia S. Kyrylenko Sergiy D. Korniienko Viktoriya V. Pogorielov Maksym V. Roshchupkin Anton A. Yanovska Hanna O. popup.nrat_date 2022-12-25 Close