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Information × Registration Number 0223U000131, 0121U100472 , R & D reports Title Development of intraoperative diagnosis method for malignant tumors determining using fluorophore-conjugated antibodies to carcinoembryonic antigens popup.stage_title Head Lyndin Mykola S., Кандидат медичних наук Registration Date 03-01-2023 Organization Sumy State University popup.description2  The object of research is malignant tumors of the main localizations. The subject of research is the immunophenotype of malignant tumor cells, molecular features of the interaction of fluorophore-conjugated antibodies with cancer cells in vitro and in vivo, the effectiveness of fluorescence-guided surgery in the visualization of malignant neoplasms. The aim of the work is to create a technique of intraoperative photodynamic imaging in the near infrared region of the spectrum of malignant tumors of the main localizations using fluorophore-conjugated antibodies to molecules of cancer-embryonic antigen. To achieve this goal, the following tasks were identified: to investigate in vitro interaction of fluorophore-conjugated anti-CEACAM antibodies with malignant tumors cells; to establish the features of interaction of fluorophore-conjugated anti-CEACAM antibodies with different types of tumor cells; to determine the optimal concentrations of fluorophore-conjugated anti-CEACAM antibodies for the indication of neoplastic cells; to investigate the stability of the "cell-fluorescent label" complex. Research methods – histological (hematoxylin and eosin staining) and immunofluorescence (determination of CEACAM expression) study of postoperative material; creation of fluorophore-conjugated anti-CEACAM antibodies according to the protocols of the fluorophore manufacturer; mathematical methods. Research is aimed at establishing the immunophenotype of tumor tissue, determining the influence of cell adhesion molecules on the morphological and molecular genetic features of carcinomas Product Description popup.authors Hyriavenko Nataliia І. Deineka Volodymyr M. Zolotareva Vira H. Kravchenko Yaroslav O. Kuzenko Yevhen V. Lyndin Mykola S. Lyndina Yuliia M. Moskalenko Roman A. Oleshko Oleksandr M. Oleshko Tetiana B. Roshchupkin Anton A. Sikora Yana V. Sikora Vladyslav V. Sikora Kateryna O. Soroka Yuliya A. Tsepochko Dariya H. Yanko Illya K. popup.nrat_date 2023-01-03 Close